Anti-HLA Class I Purified

Anti-HLA Class I Purified

• Background: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
• Specifications: The antibody MEM-147 reacts with an extracellular epitope of all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: PHA-activated peripheral blood lymphocytes
• Target Antigen: HLA Class I
• Isotype: IgG1
• Clone: MEM-147
• Applications: Flow cytometry: Recommended dilution: 1-5 μg/mL. Western blotting: Non-reducing conditions.
• Validated Applications: FC, IP, WB
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 1591(10):3901-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9573028?itool=,*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May19(5):675-84., URL: http://www.ncbi.nlm.nih.gov/pubmed/17446208?itool=,*Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug53(6):440-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/11685454?dopt=,
• Storage Conditions: Store at 2-8°C. Do not freeze.