Anti-TNF alpha Purified

Anti-TNF alpha Purified

• Background: TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
• Specifications: The mouse monoclonal antibody MAb1 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human TNF-alpha
• Target Antigen: TNF alpha
• Isotype: Igg1 κ
• Clone: MAb1
• Applications: ELISA: It can be used as capture antibody in combination with biotinylated antibody MAb11.
• Validated Applications: WB, ELISA
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Cesaro-Tadic S, Dernick G, Juncker D, Buurman G, Kropshofer H, Michel B, Fattinger C, Delamarche E: High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems. Lab Chip. 2004 Dec4(6):563-9., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=dernick%20g%20juncker%20d ,*Visser J, Graffelman W, Blauw B, Haspels I, Lentjes E, de Kloet ER, Nagelkerken L: LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone. J Neuroimmunol. 2001 Oct 1119(2):343-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/11585638 ,*Yan SR, Qing G, Byers DM, Stadnyk AW, Al-Hertani W, Bortolussi R: Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide. Infect Immun. 2004 Mar72(3):1223-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/14977922 ,*Wahlström J, Katchar K, Wigzell H, Olerup O, Eklund A, Grunewald J: Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis. Am J Respir Crit Care Med. 2001 Jan163(1):115-21., URL: http://www.ncbi.nlm.nih.gov/pubmed/11208635 ,*Lindholm C, Naylor A, Johansson EL, Quiding-Järbrink M: Mucosal vaccination increases endothelial expression of mucosal addressin cell adhesion molecule 1 in the human gastrointestinal tract. Infect Immun. 2004 Feb72(2):1004-9., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=lindholm%20c%20naylor%20a ,*Ahlstedt I, Lindholm C, Lönroth H, Hamlet A, Svennerholm AM, Quiding-Järbrink M: Role of local cytokines in increased gastric expression of the secretory component in Helicobacter pylori infection. Infect Immun. 1999 Sep67(9):4921-5., URL: http://www.ncbi.nlm.nih.gov/pubmed/10456951,
• Storage Conditions: Store at 2-8°C. Do not freeze.