Anti-alpha-Tubulin Purified

Anti-alpha-Tubulin Purified

• Background: The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening 8211 this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
• Specifications: The rat monoclonal antibody YOL1/34 recognizes an epitope of alpha-tubulin localized between amino acids 414-422. It has higher affinity for fixed microtubules than for native ones.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Mouse, Rat, Mammalian, Avian, Yeast, Human
• Immunogen: Yeast tubulin
• Target Antigen: alpha-Tubulin
• Isotype: IgG2a
• Clone: YOL1/34
• Applications: Immunocytochemistry: Recommended dilution 4-8 μg/mL.Western blotting: Recommended dilution 1-2 μg/mL reducing conditions.Flow cytometry: Recommended dilution: 8-12 μg/mL intracellular staining.
• Validated Applications: IHC-F, FC-IC, ELISA, WB, IHC-P
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Badin-Larçon AC, Boscheron C, Soleilhac JM, Piel M, Mann C, Denarier E, Fourest-Lieuvin A, Lafanechère L, Bornens M, Job D: Suppression of nuclear oscillations in Saccharomyces cerevisiae expressing Glu tubulin. Proc Natl Acad Sci U S A 2004 Apr 13101(15):5577-82, URL: https://pubmed.ncbi.nlm.nih.gov/15031428/,*Harris GS, Keath EJ, Medoff J: Expression of alpha- and beta-tubulin genes during dimorphic-phase transitions of Histoplasma capsulatum. Mol Cell Biol. 1989 May9(5):2042-9, URL: https://pubmed.ncbi.nlm.nih.gov/2546058/,*Larcher JC, Boucher D, Lazereg S, Gros F, Denoulet P: Interaction of kinesin motor domains with alpha- and beta-tubulin subunits at a tau-independent binding site. Regulation by polyglutamylation. J Biol Chem. 1996 Sep 6271(36):22117-24, URL: https://pubmed.ncbi.nlm.nih.gov/8703022/,*Breitling F, Little M: Carboxy-terminal regions on the surface of tubulin and microtubules. Epitope locations of YOL1/34, DM1A and DM1B. J Mol Biol. 1986 May 20189(2):367-70, URL: https://pubmed.ncbi.nlm.nih.gov/2427729/,
• Storage Conditions: Store at 2-8°C. Do not freeze.