Anti-alpha-Fetoprotein Purified

Anti-alpha-Fetoprotein Purified

• Background: Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
• Specifications: The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: purified alpha-Fetoprotein
• Target Antigen: alpha-Fetoprotein
• Isotype: IgG1
• Clone: AFP-11
• Applications: ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100). Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL positive tissue: hepatocellular carcinoma heat mediated antigen retrieval (sodium citrate buffer) mAb incubation 1 hour / RT, detection DAB.Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions.
• Validated Applications: IHC-P, ELISA, RIA, WB, ICC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28111(1):67-73., URL: https://pubmed.ncbi.nlm.nih.gov/2455753/,
• Storage Conditions: Store at 2-8°C. Do not freeze.