Anti-TNF alpha FITC

• Description:

  TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.

• Certification:

  RUO

• Immunogen:

  Recombinant human TNF-alpha

• Target Antigen:

  TNF alpha

• Clone:

  MAb11

• Applications:

  FC-IC (QC tested)

• Format:

  FITC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Cesaro-Tadic S, Dernick G, Juncker D, Buurman G, Kropshofer H, Michel B, Fattinger C, Delamarche E: High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems. Lab Chip. 2004 Dec;4 (6) :563-9., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=dernick%20g%20juncker%20d , *Attarbaschi T, Willheim M, Ramharter M, Hofmann A, Wahl K, Winkler H, Graninger W, Winkler S: T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis) . Eur Cytokine Netw. 2003 Jan-Mar;14 (1) :34-9., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=attarbaschi%20t%20willheim , *Wahlström J, Katchar K, Wigzell H, Olerup O, Eklund A, Grunewald J: Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis. Am J Respir Crit Care Med. 2001 Jan;163 (1) :115-21., URL: http://www.ncbi.nlm.nih.gov/pubmed/11208635, *Yan SR, Qing G, Byers DM, Stadnyk AW, Al-Hertani W, Bortolussi R: Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide. Infect Immun. 2004 Mar;72 (3) :1223-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/14977922 , *Visser J, Graffelman W, Blauw B, Haspels I, Lentjes E, de Kloet ER, Nagelkerken L: LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone. J Neuroimmunol. 2001 Oct 1;119 (2) :343-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/11585638

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha) .

• Applications Notes:

  6

• Species:

  Pig, Human, Non-human primates

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa