USP10 antibody

• Background :

  Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989) .

• Synonyms :

  Ubiquitin carboxyl-terminal hydrolase 10|Deubiquitinating enzyme 10|Ubiquitin thioesterase 10|Ubiquitin-specific-processing protease 10|USP10|KIAA0190

• Gene ID :

  9100

• UniProt :

  Q14694

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  Ubiquitin specific peptidase 10

• Target :

  USP10

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, IHC, IF

• Field of Research :

  Epigenetics, Metabolism

• Purification :

  Immunogen affinity purified

• Dilution :

  IHC: 1:50-1:200; IF: 1:20-1:100

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, IHC, IF