USP13 antibody

• Background:

  Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD) . Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD) . Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.

• Synonyms:

  Ubiquitin carboxyl-terminal hydrolase 13|Deubiquitinating enzyme 13|Isopeptidase T-3 (ISOT-3) |Ubiquitin thioesterase 13|Ubiquitin-specific-processing protease 13|USP13|ISOT3

• Gene ID:

  8975

• UniProt:

  Q92995

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Ubiquitin specific peptidase 13 (isopeptidase T-3)

• Target:

  USP13

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Applications:

  ELISA, WB, IHC, IF

• Field of Research:

  Epigenetics, Metabolism

• Purification:

  Protein A+G purification

• Dilution:

  WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  97 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID:

  9E6

• Tested Applications:

  ELISA, WB, IHC, IF