USP15 antibody

• Background :

  Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling and NF-kappa-B pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed:21947082) . According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal (PubMed:22344298) . Able to mediate deubiquitination of monoubiquitinated substrates as well as 'Lys-48'-linked polyubiquitin chains, protecting them against proteasomal degradation. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B (PubMed:24526689) . Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.

• Synonyms :

  Ubiquitin carboxyl-terminal hydrolase 15|Deubiquitinating enzyme 15|Ubiquitin thioesterase 15|Ubiquitin-specific-processing protease 15|Unph-2|Unph4|USP15|KIAA0529

• Gene ID :

  9958

• UniProt :

  Q9Y4E8

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Ubiquitin specific peptidase 15

• Target :

  USP15

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB, IP

• Field of Research :

  Epigenetics, Metabolism

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:500-1:5000; IP: 1:500-1:5000

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  112 kDa

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB, IP