USP16 antibody

• Background :

  Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B-and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.

• Synonyms :

  Ubiquitin carboxyl-terminal hydrolase 16|Deubiquitinating enzyme 16|Ubiquitin thioesterase 16|Ubiquitin-processing protease UBP-M|Ubiquitin-specific-processing protease 16|USP16

• Gene ID :

  10600

• UniProt :

  Q9Y5T5

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Ubiquitin specific peptidase 16

• Target :

  USP16

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB, IHC, IP

• Field of Research :

  Epigenetics, Metabolism

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:20-1:200

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  94 kDa

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB, IHC, IP