Mouse anti Phosphotyrosine, conjugated with FITC

Mouse anti Phosphotyrosine, conjugated with FITC

• Background: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
• Synonyms: Mouse Anti phosphotyrosine, PY20, FITC conjugate
• CAS Number: 9007-83-4
• Host: Mouse
• Isotype: IgG2b
• Clone: PY20
• Conjugation: FITC
• Type: Monoclonal Antibody
• Source: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
• Applications: WB, IP, Enzyme Immunoassay, IF
• Field of Research: Signal Transduction
• Purification Method: Prot A/G
• Assay Principle: A dilution of 5-10 µg/mL is sµggested for immunohistochemistry or immunofluorescence. Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as sterile solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, 3 mM sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• Shipping Conditions: Ambient Temperature
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: IF