Mouse anti Phosphotyrosine, conjugated with biotin

Mouse anti Phosphotyrosine, conjugated with biotin

• Background: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated proteins biochemical pathways in which they function.
• Synonyms: Mouse Anti phosphotyrosine, PY20
• Host: Mouse
• Isotype: IgG2b
• Clone: PY20
• Conjugation: Biotin
• Type: Monoclonal Antibody
• Source: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
• Applications: WB, IP, Enzyme Immunoassay, IHC
• Field of Research: Signal Transduction
• Purification Method: Prot A/G
• Assay Principle: The biotin conjµgate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce unacceptable background signals. In addition, the strength of the biotin-streptavidin interaction and the immense variety of streptavidin-coupled probes available make PY20-biotin a very versatile reagent._x000D_ _x000D_ A dilution of 1:5,000 is sµggested for Western blot or ELISA, 1:500 for immunohistochemistry or immunocytochemistry.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in PBS with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals._x000D_ _x000D_ This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Shipping Conditions: Ambient Temperature
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB