Mouse anti Phosphotyrosine, conjugated with peroxidase

Mouse anti Phosphotyrosine, conjugated with peroxidase

• Background: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The peroxidase conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce undesirable background signals.
• Synonyms: Mouse Anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate
• Host: Mouse
• Isotype: IgG2b
• Clone: PY20
• Conjugation: HRP Conjugate
• Type: Monoclonal Antibody
• Applications: WB, IP, IHC, Enzyme Immunoassay
• Field of Research: Signal Transduction
• Purification Method: Prot A/G
• Assay Principle: Application: A dilution of 1:2,000 is sµggested for western blot or ELISA, 1:500 for immunoprecipitation, immunohistochemistry, or immunocytochemistry.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided in 60% glycerol, phosphate buffered saline, and 0,1% 2-Chloroacetamide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• Shipping Conditions: Room Temperature
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB