IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit

• Description:

  The IkappaB-alpha (Phospho-Tyr305) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IkappaB-alpha (Phospho-Tyr305) protein expression profile in cells. The kit can be used for measuring the relative amounts of IkappaB-alpha (Phospho-Tyr305) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IkappaB-alpha (Phospho-Tyr305) .

• Synonyms:

  I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1

• Gene Name:

  NFKBIA

• UniProt:

  P25963

• Reactivity:

  Human, Mouse, Rat

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

• Molecular Weight:

  35609 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  NF-kappa-B inhibitor alpha

• Subcellular Location:

  Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.