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Trk A Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1576Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1576Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Trk A Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Trk A protein expression profile in cells. The kit can be used for measuring the relative amounts of Trk A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Trk A.
Synonyms
High affinity nerve growth factor receptor;2.7.10.1 ; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A; Trk-A; gp140trk ; p140-TrkA; NTRK1; MTC, TRK , TRKA
Gene Name
NTRK1MTC, TRK , TRKA
UniProt
P04629
Reactivity
Human, Mouse, Rat
Tissue Specificity
Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1850821, PubMed:1849459, PubMed:1281417, PubMed:8325889, PubMed:15488758, PubMed:17196528, PubMed:27445338) . Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity) . Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417) . Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
Molecular Weight
87497 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
High affinity nerve growth factor receptor
Subcellular Location
Cell membrane; Single- pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein. Rapidly internalized after NGF binding (PubMed:1281417) . Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes.

UniProtKB · P04629

High affinity nerve growth factor receptor

NTRK1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04629
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NTRK1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.10.1
Processing
Precursor
Secondary accessions
B2R6T5, B7ZM34, P08119, Q15655, Q15656, Q5D056, Q5VZS2, Q7Z5C3, Q9UIU7
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneEndosomeMembrane

Molecular function

Developmental proteinKinaseReceptorTransferaseTyrosine-protein kinase

Biological process

DifferentiationNeurogenesis

Disease

Disease variantProto-oncogene

PTM

Disulfide bondGlycoproteinPhosphoproteinUbl conjugation

Domain

Immunoglobulin domainLeucine-rich repeatRepeatSignalTransmembraneTransmembrane helix

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