RAD51 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1493Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No

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CAT#:0519-EKC1493Size:1 Kit, containing one 96 Well Plate and all necessary reagents

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

The RAD51 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RAD51 protein expression profile in cells. The kit can be used for measuring the relative amounts of RAD51 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RAD51.

Synonyms

DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A; RAD51; RAD51A, RECA

Gene Name

RAD51

UniProt

Q06609

Reactivity

Human, Mouse

Tissue Specificity

Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:28575658) . Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308) . Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028) .

Molecular Weight

36966 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

DNA repair protein RAD51 homolog 1

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.

UniProtKB · Q06609

DNA repair protein RAD51 homolog 1

RAD51_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q06609
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: RAD51
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: HsRAD51, hRAD51
EC number: 3.6.4.-
Processing: —
Secondary accessions: B0FXP0, B2R8T6, Q6FHX9, Q6ZNA8, Q9BV60

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

ChromosomeCytoplasmCytoskeletonMitochondrionNucleus

Disease

Disease variantFanconi anemia

Biological process

DNA damageDNA recombinationDNA repair

Molecular function

DNA-bindingHydrolase

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