RAD51 (Phospho-Tyr315) Colorimetric Cell-Based ELISA Kit

• Description:

  The RAD51 (Phospho-Tyr315) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RAD51 (Phospho-Tyr315) protein expression profile in cells. The kit can be used for measuring the relative amounts of RAD51 (Phospho-Tyr315) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RAD51 (Phospho-Tyr315) .

• Synonyms:

  DNA repair protein RAD51 homolog 1; RAD51A; RECA

• Gene Name:

  RAD51

• UniProt:

  Q06609

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:28575658) . Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308) . Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028) .

• Molecular Weight:

  36966 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  DNA repair protein RAD51 homolog 1

• Subcellular Location:

  Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.