PP2A-alpha Colorimetric Cell-Based ELISA Kit

• Description:

  The PP2A-alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PP2A-alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of PP2A-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PP2A-alpha.

• Synonyms:

  Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha;3.1.3.16; Replication protein C; RP-C; PPP2CA

• Gene Name:

  PPP2CA

• UniProt:

  P67775

• Reactivity:

  Human, Mouse, Rat

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  PP2A is the major phosphatase for microtubule-associated proteins (MAPs) . PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity) . Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

• Molecular Weight:

  35594 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

• Subcellular Location:

  Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGO2 (By similarity) .