MAP3K8 Colorimetric Cell-Based ELISA Kit

• Description:

  The MAP3K8 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MAP3K8 protein expression profile in cells. The kit can be used for measuring the relative amounts of MAP3K8 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MAP3K8.

• Synonyms:

  COT proto-oncogene serine/threonine-protein kinase; C-COT; Cancer Osaka thyroid oncogene; Tumor progression locus 2; TPL-2

• Gene Name:

  MAP3K8

• UniProt:

  P41279

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed in several normal tissues and human tumor-derived cell lines.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Required for lipopolysaccharide (LPS) -induced, TLR4- mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF- alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B- cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

• Molecular Weight:

  52925 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Mitogen-activated protein kinase kinase kinase 8

• Subcellular Location:

  Cytoplasm.