MAP3K8 (Phospho-Thr290) Colorimetric Cell-Based ELISA Kit

• Description:

  The MAP3K8 (Phospho-Thr290) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MAP3K8 (Phospho-Thr290) protein expression profile in cells. The kit can be used for measuring the relative amounts of MAP3K8 (Phospho-Thr290) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MAP3K8 (Phospho-Thr290) .

• Synonyms:

  C-COT; COT proto-oncogene serine/threonine-protein kinase; Cancer Osaka thyroid oncogene; M3K8; MAP3K8; Mitogen-activated protein kinase kinase kinase 8; TPL-2; TPL2; Tpl2; Tumor progression locus 2; kinase Cot

• Gene Name:

  MAP3K8

• UniProt:

  P41279

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed in several normal tissues and human tumor-derived cell lines.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Required for lipopolysaccharide (LPS) -induced, TLR4- mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF- alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B- cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

• Molecular Weight:

  52925 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Mitogen-activated protein kinase kinase kinase 8

• Subcellular Location:

  Cytoplasm.