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INOS Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1302Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1302Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The iNOS Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor iNOS protein expression profile in cells. The kit can be used for measuring the relative amounts of iNOS in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on iNOS.
Synonyms
Nitric oxide synthase inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOS2; NOS2A
Gene Name
NOS2
UniProt
P35228
Reactivity
Human
Tissue Specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004) . In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity) . As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112) . Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109) .
Molecular Weight
131117 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Nitric oxide synthase, inducible

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