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GATA1 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1236Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1236Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The GATA1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor GATA1 protein expression profile in cells. The kit can be used for measuring the relative amounts of GATA1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on GATA1.
Synonyms
Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein; GATA1; ERYF1; GF1
Gene Name
GATA1
UniProt
P15976
Reactivity
Human, Mouse, Rat
Tissue Specificity
Erythrocytes.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781) .
Molecular Weight
42751 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Erythroid transcription factor
Subcellular Location
Nucleus.

UniProtKB · P15976

Erythroid transcription factor

GATA1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15976
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GATA1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q96GB8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Molecular function

ActivatorDNA-bindingRepressor

Coding sequence diversity

Alternative initiationAlternative splicing

Disease

Disease variantHereditary hemolytic anemia

Ligand

Metal-bindingZinc

Cellular component

Nucleus

Domain

RepeatZinc-finger

Biological process

TranscriptionTranscription regulation

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