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Doublecortin Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1174Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1174Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Doublecortin Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Doublecortin protein expression profile in cells. The kit can be used for measuring the relative amounts of Doublecortin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Doublecortin.
Synonyms
DBCN; DCX; Doublin; Lis-X; Lissencephalin-X; LISX
Gene Name
DCX
UniProt
O43602
Reactivity
Human, Mouse, Rat
Tissue Specificity
Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Molecular Weight
40574 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Neuronal migration protein doublecortin
Subcellular Location
Cytoplasm. Cell projection. Localizes at neurite tips.

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