Caspase 3 Colorimetric Cell-Based ELISA Kit

• Description:

  The Caspase 3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Caspase 3 protein expression profile in cells. The kit can be used for measuring the relative amounts of Caspase 3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Caspase 3.

• Synonyms:

  Apopain precursor; CASP-3; CPP-32; CPP32; Caspase-3; Cysteine protease CPP32; ICE3; SCA-1; SREBP cleavage activity 1; Yama protein

• Gene Name:

  CASP3

• UniProt:

  P42574

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

• Molecular Weight:

  31608 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Caspase-3

• Subcellular Location:

  Cytoplasm.