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BID Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1059Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1059Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The BID Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor BID protein expression profile in cells. The kit can be used for measuring the relative amounts of BID in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BID.
Synonyms
BH3 interacting domain death agonist
Gene Name
BID
UniProt
P55957
Reactivity
Human, Mouse
Tissue Specificity
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level) . Isoform 3 is expressed in lung, pancreas and spleen (at protein level) . Isoform 4 is expressed in lung and pancreas (at protein level) .
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity) . Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Molecular Weight
21995 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
BH3-interacting domain death agonist
Subcellular Location
Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic.

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