BID Colorimetric Cell-Based ELISA Kit

• Description:

  The BID Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor BID protein expression profile in cells. The kit can be used for measuring the relative amounts of BID in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BID.

• Synonyms:

  BH3 interacting domain death agonist

• Gene Name:

  BID

• UniProt:

  P55957

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level) . Isoform 3 is expressed in lung, pancreas and spleen (at protein level) . Isoform 4 is expressed in lung and pancreas (at protein level) .

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  The major proteolytic product p15 BID allows the release of cytochrome c (By similarity) . Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

• Molecular Weight:

  21995 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  BH3-interacting domain death agonist

• Subcellular Location:

  Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic.