ATF5 Colorimetric Cell-Based ELISA Kit

• Description:

  The ATF5 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ATF5 protein expression profile in cells. The kit can be used for measuring the relative amounts of ATF5 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ATF5.

• Synonyms:

  Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx; ATF5; ATFX

• Gene Name:

  ATF5

• UniProt:

  Q9Y2D1

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Widely expressed with higher expression levels in liver.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'- GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631) . Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (PubMed:15358120) . Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate (By similarity) . Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation (By similarity) . Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes (By similarity) . May be involved in osteogenic differentiation (PubMed:22442021) . Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (PubMed:21791614) . Plays an anti- apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent (By similarity) . Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver (PubMed:18332083) . In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase (PubMed:22528486, PubMed:18701499) . May act as a negative regulator of IL1B transduction pathway in liver (PubMed:24379400) . Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613) . Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation (PubMed:24216764) . Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (PubMed:26213385) .

• Molecular Weight:

  30674 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Cyclic AMP-dependent transcription factor ATF-5

• Subcellular Location:

  Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, translocates from the centrosome to the midbody.