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HSL Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1285Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1285Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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Description
The HSL Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSL protein expression profile in cells. The kit can be used for measuring the relative amounts of HSL in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSL.
Synonyms
Hormone sensitive lipase; LIPE; LIPS
Gene Name
LIPE
UniProt
Q05469
Reactivity
Human, Mouse, Rat
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Molecular Weight
116598 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Hormone-sensitive lipase
Subcellular Location
Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.

UniProtKB · Q05469

Hormone-sensitive lipase

LIPS_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q05469
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LIPE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
HSL
EC number
3.1.1.79
Processing
—
Secondary accessions
Q3LRT2, Q6NSL7
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCytoplasmLipid dropletMembrane

Biological process

Cholesterol metabolismLipid degradationLipid metabolismSteroid metabolismSterol metabolism

Disease

Diabetes mellitusObesity

Molecular function

Hydrolase

PTM

Phosphoprotein

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