OCT3 Colorimetric Cell-Based ELISA Kit

• Description:

  The OCT3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor OCT3 protein expression profile in cells. The kit can be used for measuring the relative amounts of OCT3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on OCT3.

• Synonyms:

  POU domain class 5 transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3; POU5F1; OCT3; OCT4; OTF3

• Gene Name:

  POU5F1

• UniProt:

  Q01860

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

• Molecular Weight:

  38571 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  POU domain, class 5, transcription factor 1

• Subcellular Location:

  Cytoplasm. Nucleus. Expressed in a diffuse and slightly punctuate pattern.