Mouse CCL7/MCP-3 ELISA Kit PicoKine®

Mouse CCL7/MCP-3 ELISA Kit PicoKine®

• Background: Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
• Description: Mouse CCL7/MCP-3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: C-C motif chemokine 7;Intercrine/chemokine MARC;Monocyte chemoattractant protein 3;Monocyte chemotactic protein 3;MCP-3;Protein FIC;Small-inducible cytokine A7;Ccl7;Fic, Mcp3, Scya7;
• CAS Number: 7732-18-5
• Gene Name: C-C motif chemokine 7
• Gene ID: 20306
• UniProt: Q03366
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: Q24-P97
• Applications: ELISA
• Standard: 10 ng/tube
• Sensitivity: <1 pg/ml
• Function: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity (By similarity). .
• : heparin or EDTA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 10999 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	33	34	32	32	32	0.82	2.5%
  Sample 2	194	218	205	216	208	9.6	4.6%
  Sample 3	459	441	412	484	449	26.25	5.8%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse CCL7
• Subcellular Location: Secreted.
• Protein Name: C-C motif chemokine 7