Human CCL7/MCP-3 PicoKine® Quick ELISA Kit

Human CCL7/MCP-3 PicoKine® Quick ELISA Kit

• Background: Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
• Description: Human CCL7/MCP-3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• CAS Number: 7732-18-5
• Gene Name: chemokine (C-C motif) ligand 7
• Gene ID: 6354
• UniProt: P80098
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: Q24-L99
• Applications: ELISA
• Standard: 10 ng/tube
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Components: Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
• : heparin or EDTA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <1 pg/ml
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	35	31	36	41	36	3.32	9.3%
  Sample 2	175	166	170	169	170	2.25	1.5%
  Sample 3	512	526	443	429	478	39.63	8.3%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant Human CCL7
• Protein Name: C-C motif chemokine 7