ERK2 Antibody / MAPK1

ERK2 Antibody / MAPK1

• Description: Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely-related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development.
• UniProt: P28482
• Host: Mouse
• Immunogen: Recombinant full-length human protein was used as the immunogen for the ERK2 antibody.
• Clonality: Monoclonal
• Isotype: IgG2b
• Applications: ELISA, FACS, IF
• Format: Purified
• Buffer: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Reconstitution: Aliquot the ERK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
• Limitations: This ERK2 antibody is available for research use only.