RecombinantEndostatin, Human

• Description:

  Endostatin is a cleaved product of the carboxyl-terminal domain of collagen XVIII. It functions as an anti-angiogenic cytokine that is expressed in various organs especially with high levels in liver, lung, and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant Endostatin expressed in yeast, whichcauses G1 arrest of endothelial cells. Endostatin treatment results in apoptosis of HUVE and HMVE cells. Recombinant human Endostatin is a 20.2 kDa protein consisting of 184 amino acid residues.

• Endotoxin:

  Endotoxin level is less than 0.1 ng per μg (1EU/μg) of Endostatin.

• Purity:

  Greater than 97% by SDS-PAGE

• Bioactivity:

  Determined by Endothelial Cell Tube Formation. The cells treated with 10μ g/ml Endostatin showed significant anti-angiogenesis effect.

• Reconstitution:

  Reconstituted in ddH2O or PBS at 100 μg/ml.

• Molecular Weight:

  ~20.2 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  The lyophilized Endostatin is stable for a few weeks at room temperature, but best stored at -20°C. For the first use, reconstitute in water, aliquot and store at -20°C to avoid multiple freeze-thaws.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  The sterile filtered solution was lyophilized with 17 mM citric-phosphate buffer, pH 6.2.

• Host or Source:

  P. pastoris

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  AH SHRDFQPVLH LVALNSPLSG GMRGIRGADF QCFQQARAVG LAGTFRAFLS SRLQDLYSIV RRADRAAVPI VNLKDELLFP SWEALFSGSE GPLKPGARIF SFDGKDVLRH PTWPQKSVWH GSDPNGRRLT ESYCETWRTE APSATGQASS LLGGRLLGQS AASCHHAYIV LCIENSFMTA SK