RecombinantVEGF165, Human

• Description:

  Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.Recombinant human Vascular Endothelial Growth Factor A165 (rhVEGF-A165) produced in Pichia pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rhVEGF-A165 has a molecular mass of 38.2kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

• Endotoxin:

  <0.5 EU/μg, determined by LAL method.

• Purity:

  > 95% as analyzed by reducing SDS-PAGE.

• Bioactivity:

  ED50 of 1-5ng/ml, measured by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of 2x 10ˆ5-1x 10ˆ6 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/ml.

• Molecular Weight:

  38.2kDa, observed by non-reducing SDS-PAGE

• Storage Conditions:

  Lyophilized recombinant human Vascular Endothelial Growth Factor A165 (rhVEGF-A165) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhVEGF-A165 should be stable up to 4 week at 4°C or up to 6 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0.

• Host or Source:

  P. pastoris

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  APMAEG GGQNHHEVVK FMDVYQRSYC HPIETLVDIF QEYPDEIEYI FKPSCVPLMR CGGCCNDEGL ECVPTEESNI TMQIMRIKPH QGQHIGEMSF LQHNKCECRP KKDRARQENP CGPCSERRKH LFVQDPQTCK CSCKNTDSRC KARQLELNER TCRCDKPRR