RecombinantDHH, Mouse

• Description:

  Desert hedgehog protein (DHH) is a member of the Hedgehog family which encodes signaling molecules that play an important role in regulating morphogenesis. It is predicted to be made as a precursor that is auto-catalytically cleaved; the N-terminal portion is soluble and contains the signaling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. DHH may be involved in both male gonadal differentiation and perineurial development. DHH binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) . It induces steroidogenicfactor 1 (SF1), which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules.Recombinant mouse Desert Hedgehog (DHH) produced in E.coli is a single non-glycosylated polypeptide chain containing 176 amino acids. A fully biologically active molecule, rmDHH has a molecular mass of 20.1kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 98% as analyzed by SDS-PAGE and HPLC.

• Bioactivity:

  ED50 <20 μg/ml, measured by its ability to induce alkaline phosphatase production by CCL-226 cells.

• Reconstitution:

  Reconstituted in ddH2O or PBS at 100 μg/ml.

• Molecular Weight:

  20.1 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant Mouse Desert Hedgehog (DHH) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse Desert Hedgehog (DHH) should be stable up to 1 week at 4°C or up to 2 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  IIG PGRGPVGRRR YVRKQLVPLL YKQFVPSMPE RTLGASGPAE GRVTRGSERF RDLVPNYNPD IIFKDEENSG ADRLMTERCK ERVNALAIAV MNMWPGVRLR VTEGWDEDGH HAQDSLHYEG RALDITTSDR DRNKYGLLAR LAVEAGFDWV YYESRNHIHV SVKADNSLAV RAGG