Products for Research Use Only
RegisterLogin

RecombinantShh (C24II), Human

CAT: 0384-BK0159-01Size: 10 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0384-BK0159-01Size:10 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) . They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical) . Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant human Sonic Hedgehog (C24II) (rhShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 175 amino acids. A fully biologically active molecule, rhShh has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 < 2.0 μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 500 units/mg.
Reconstitution
Reconstituted in ddH2O at 100 μg/ml.
Molecular Weight
19.7 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant human Sonic Hedgehog (C24II) (rhShh) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhShh should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
IIG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT LGASGRYEGK ISRNSERFKE LTPNYNPDII FKDEENTGAD RLMTQRCKDK LNALAISVMN QWPGVKLRVT EGWDEDGHHS EESLHYEGRA VDITTSDRDR SKYGMLARLA VEAGFDWVYY ESKAHIHCSV KAENSVAAKS GG

Related Products

CatalogName
BK0160-01RecombinantShh (C24IVI), Human
BK0162-01RecombinantShh, Mouse
BK0161-01RecombinantShh (C25II), Mouse
BK0284-01RecombinantShh, Mouse (CHO-expressed)
BK0021-01RecombinantDHH, Human
BK0088-01RecombinantIHH, Human
BK0063-01RecombinantGH, Human
BK0218-01RecombinantHB-EGF, Human
BK0073-01RecombinantHB-EGF, Human
BK0357-01RecombinantsIL-6Rα, His, Human

Alternative Products

CatalogName
GTR18990462-01RecombinantShh (C24II), Human
BK0159RecombinantShh(C24II),Human
GTR18990461-01RecombinantShh (C24IVI), Human
BK0160RecombinantShh(C24IVI),Human
Z03067-50Shh (C24II), Human
OM297284-01SHH (C24II) Human
HY-P7407-01SHH, Human (C24II)
PROTQ15465-2SHH (C24II)Human Sonic HedgeHog (C24II) Human Recombinant Protein
C089-01Recombinant Human SHH (C24II)
UA040095-01Shh (C24II) Protein, Human

Popular Products