RecombinantIHH, Human

CAT: 0384-BK0088-01Size: 10 µgDry Ice: NoHazardous: No
CAT#:0384-BK0088-01Size:10 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Indian Hedgehog protein (IHH) is one of three proteins in the mammalian hedgehog family, the others being desert hedgehog (DHH) and Sonic hedgehog (SHH) . Hedgehog proteins are important signaling molecules during embryonic development and are highly conserved across species. Mouse and human IHH share 100% amino acid identity in the signaling domain, while mouse IHH and SHH share 90% amino acid identity in the N-terminal signaling domain. IHH mRNA expression is detected in fetal lung, gut, stomach, liver, kidney, pancreas and strongly in cartilage in growth regions of the developing bone. IHH has a specific role in bone growth and differentiation. In addition, IHH is involved in yolk sac vasculogenesis, having a central role in differentiation of epiblast cells into endothelial and red blood cells. IHH gene mutations cause the brachydactyly type A1 which is characterized by shortening or malformation of the phalanges and also the acrocapitofemoral dysplasia.Recombinant Human Indian Hedgehog (IHH) produced in E.coli is a single non-glycosylated polypeptide chain containing 178 amino acids. A fully biologically active molecule, rhIHH has a molecular mass of 20 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE.
Bioactivity
ED50 <3μg/ml, measure d by its ability to induce alkaline phosphatase production by CCL-226 cells.
Reconstitution
Reconstituted in ddH2O or PBS at 100 μg/ml.
Molecular Weight
20 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant Human Indian Hedgehog (IHH), remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human IHH should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
IIG PGRVVGSRRR PPRKLVPLAY KQFSPNVPEK TLGASGRYEG KIARSSERFK ELTPNYNPDI IFKDEENTGA DRLMTQRCKD RLNSLAISVM NQWPGVKLRV TEGWDEDGHH SEESLHYEGR AVDITTSDRD RNKYGLLARL AVEAGFDWVY YESKAHVHCS VKSEHSAAAK TGG

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