RecombinantIFN-γ, Rat

• Description:

  Interferon gamma (IFN-γ), also known as Type II interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and activates the IFN-γ/JAK/STAT pathway. IFN-γ signaling promotesbiological functions primarily related to antiviral and antibacterial defense, apoptosis, inflammation, and regulation of innate and acquired immune responses. While IFN-γ–induced inflammatory cascades summon a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.Recombinant Rat Interferon gamma (IFN-γ) produced in E.coli is a single non-glycosylated polypeptide chain containing 134 amino acids. A fully biologically active molecule, rrIFN-γ has a molecular mass of 15.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% as analyzed by SDS-PAGE and HPLC.

• Bioactivity:

  ED50 <0.5 ng/ml, measured by cytotoxicity assay using WEHI-279 cells, corresponding to a specific activity of >2x10ˆ6 units/mg.

• Reconstitution:

  Reconstituted in ddH2O or PBS at 100 μg/ml.

• Molecular Weight:

  15.5 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant Rat IFN-γ remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant Rat IFN-γ should be stable up to 1 week at 4°C or up to 2 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  GT LIESLESLKN YFNSSSMDAM EGKSLLLDIW RNWQKDGNTK ILESQIISFY LRLFEVLKDN QAISNNISVI ESHLITNFFS NSKAKKDAFM SIAKFEVNNP QIQHKAVNEL IRVIHQLSPE SSLRKRKRSR C