RecombinantIL-1β, Rat

• Description:

  Interleukin-1 Beta (IL-1 Beta) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.Recombinant rat Interleukin-1 Beta (rat IL-1 Beta) produced in E.coli is a single non-glycosylated polypeptide chain containing 152 amino acids. A fully biologically active molecule, recombinant rat IL-1 Beta protein has a molecular mass of 17.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% by SDS-PAGE and HPLC analyses.

• Bioactivity:

  ED50 < 10 pg/ml, measured by a cell proliferation assay using mouse D10S cells, corresponding to a specific activity of > 1.0x 10ˆ8 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/ml.

• Molecular Weight:

  17.3 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant rat Interleukin-1 Beta (IL-1 Beta) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant rat IL-1 Beta should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  VPI RQLHCRLRDE QQKCLVLSDP CELKALHLNG QNISQQVVFS MSFVQGETSN DKIPVALGLK GLNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKTKVEFE SAQFPNWYIS TSQAEHRPVF LGNSNGRDIV DFTMEPVSS