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RecombinantIFN-α2b, Human

CAT: 0384-BK0079-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0384-BK0079-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interferon-Alpha 2b (IFN-Alpha 2b) produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a, b, c) signaling through IFNAR2. IFN-alpha 2b shares 99.4% aa sequence identity with both IFN-alpha 2a and 2c. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.Recombinant human Interferon-Alpha 2b (rhIFN-Alpha 2b) produced in E.coli is a single non-glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rhIFN-Alpha 2b has a molecular mass of 19.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 < 0.05 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 2.0x 10ˆ7 units/mg.
Reconstitution
Reconstituted in ddH2O at 100 μg/ml.
Molecular Weight
19.2kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant human Interferon-Alpha 2b (rhIFN-Alpha 2b) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-Alpha 2b should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
CDL PQTHSLGSRR TLMLLAQMRR ISLFSCLKDR HDFGFPQEEF GNQFQKAETI PVLHEMIQQI FNLFSTKDSS AAWDETLLDK FYTELYQQLN DLEACVIQGV GVTETPLMKE DSILAVRKYF QRITLYLKEK KYSPCAWEVV RAEIMRSFSL STNLQESLRS KE

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