RecombinantIFN-α2a, Human

• Description:

  Interferon-Alpha 2a (IFN-Alpha 2a), Human produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a, b, c) signaling through IFNAR2. IFN-alpha 2a shares 99.4% , 98.8% aa sequence identity with IFN-alpha 2b and 2c respectively. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.Recombinant Interferon-Alpha 2a (IFN-Alpha 2a), Human produced in E.coli is a single non-glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rhIFN-Alpha has a molecular mass of 19.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% by SDS-PAGE and HPLC analyses.

• Bioactivity:

  ED50 < 0.1 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 1.0x 10ˆ7 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/ml.

• Molecular Weight:

  19.2kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant human Interferon-Alpha 2a (rhIFN-Alpha 2a) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-Alpha 2a should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  CDL PQTHSLGSRR TLMLLAQMRK ISLFSCLKDR HDFGFPQEEF GNQFQKAETI PVLHEMIQQI FNLFSTKDSS AAWDETLLDK FYTELYQQLN DLEACVIQGV GVTETPLMKE DSILAVRKYF QRITLYLKEK KYSPCAWEVV RAEIMRSFSL STNLQESLRS KE