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RecombinantIFN-α2a, Human

CAT: 0013-GTR18990538-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18990538-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interferon-Alpha 2a (IFN-Alpha 2a), Human produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a, b, c) signaling through IFNAR2. IFN-alpha 2a shares 99.4% , 98.8% aa sequence identity with IFN-alpha 2b and 2c respectively. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.Recombinant Interferon-Alpha 2a (IFN-Alpha 2a), Human produced in E.coli is a single non-glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rhIFN-Alpha has a molecular mass of 19.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Product Name Alternative
Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-a 2a.
Source
Escherichia coli.
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 1.0x 10ˆ7 units/mg.
Form
Lyophilized after extensive dialysis against PBS.
Molecular Weight
19.2kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant human Interferon-Alpha 2a (rhIFN-Alpha 2a) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-Alpha 2a should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O at 100 μg/ml.
Preservative
Lyophilized after extensive dialysis against PBS.
AA Sequence
CDL PQTHSLGSRR TLMLLAQMRK ISLFSCLKDR HDFGFPQEEF GNQFQKAETI PVLHEMIQQI FNLFSTKDSS AAWDETLLDK FYTELYQQLN DLEACVIQGV GVTETPLMKE DSILAVRKYF QRITLYLKEK KYSPCAWEVV RAEIMRSFSL STNLQESLRS KE

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