XRN2 polyclonal antibody

5'-3' exoribonuclease 2 (XRN2) is a nuclear exonuclease that degrades RNA containing a 5’-monophosphate to component mononucleotides. XRN2 also plays an important role in the termination of transcription at the 3’-end of genes by displacing RNA polymerase II (RNAPII) from the DNA strand . According to the ‘torpedo’ model of transcription termination, XRN2 gains access to the 5’ phosphate of the nascent RNA during co-transcriptional polyadenylation site cleavage. XRN2 degrades RNA at a faster rate than RNAPII-mediated RNA synthesis, resulting in the eviction of RNAPII from the template . In addition, XRN2 is essential for maturation of 5.8S and 28S ribosomal RNA and small nucleolar RNA molecules . Several research studies suggest that XRN2 plays a role in the quality control check of RNA molecules. XRN2 co-transcriptionally degrades aberrant nuclear mRNA transcripts that result from defective 5’mRNA capping, splicing, or 3’end formation . XRN2 exonuclease rapidly degrades hypomodified tRNA and excess miRNA molecules, indicating that XRN2 likely regulates tRNA and miRNA quality control as well .

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

5'-3' exoribonuclease 2; DHM1-like protein; DHP prote

Q9H0D6

Human, Mouse

Synthetic peptide, corresponding to amino acids 90-134 of Human XRN2

Unconjugated

WB

WB: 1:500~1:1000

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

Unmodification

~ 110 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

XRN2 polyclonal antibody detects endogenous levels of XRN2 protein.

Western blot (WB) analysis of XRN2 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (40ug)