CD52 polyclonal antibody

• Background:

  CD52 is a glycosylphosphatidylinositol (GPI) -linked surface antigen present at high levels on epithelial cells lining the male reproductive tract, thymocytes, lymphocytes, monocytes and macrophages. It is also present at variable levels on lymphoid malignancies. During sperm maturation, spermatozoa passing through the genital tract acquire CD52 that is shed from the epithelial cell lining into seminal plasma. CD52 is detectable on the surface of epididymal sperm and in the ejaculate but not on spermatogenetic cells or testicular spermatozoa. The peptide backbone of CD52, which consists of 12 amino acids, is considered a mere scaffold for posttranslational modifications, such as GPI-anchor and N-glycosylation.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  CAMPATH-1 antigen; CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; He5; CD52; CD52; CDW52, HE5

• Swiss Prot:

  P31358

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to residues in Human CD52.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 15 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD52 polyclonal antibody detects endogenous levels of CD52 protein.

• Applications Notes:

  Western blot (WB) analysis of CD52 polyclonal antibody at 1:500 dilution Lane1:THP-1 whole cell lysate Lane2:Mouse spleen tissue lysate Lane3:H9C2 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4