CD59 polyclonal antibody

• Background:

  Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  CD59 glycoprotein; 1F5 antigen; 20 kDa homologous restriction factor; HRF-20; HRF20; MAC-inhibitory protein; MAC-IP; MEM43 antigen; Membrane attack complex inhibition factor; MACIF; Membrane inhibitor of reactive lysis; MIRL; Protectin; CD59

• Swiss Prot:

  P13987

• Reactivity:

  Human

• Immunogen:

  Recombinant protein of human CD59.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:1000~1:2000 IF 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~17 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD59 polyclonal antibody detects endogenous levels of CD59 protein.

• Applications Notes:

  Western blot (WB) analysis of CD59 polyclonal antibody at 1:1000 dilution Lane1:MCF-7 cell membrane lysate (25ug) Lane2:A549 cell membrane lysate (25ug) Lane3:L02 cell membrane lysate (25ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4