CD54 polyclonal antibody

• Background :

  Intercellular cell adhesion molecule-1 (CD54 or ICAM-1) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. CD54 is expressed at low levels in diverse cell types, and is induced by cytokines (TNF-α, interleukin-1) and bacterial lipopolysaccharide. Apical localization of CD54 on endothelial cells (or basolateral localization on epithelial cells) is a prerequisite for leukocyte trafficking through the endothelial (or epithelial) barrier. Apical expression of CD54 on epithelial cells mediates pathogen invasion as well as host defense, a pattern also observed in tumors. CD54 also functions as a co-stimulator on antigen presenting cells, binding to its receptor LFA-1 (leukocyte function-associated antigen-1) on the surface of T cells during antigen presentation. Cross-linking of CD54 or binding to its ligand triggers activation of Src family kinases and the Rho/ROCK pathway. Phosphorylation on Tyr485 of CD54 is required for its association with SHP-2. SHP-2 seems essential for CD54-induced Src activation.

• CAS Number :

  9007-83-4

• Structure Composition :

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative :

  Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54

• Swiss Prot :

  P05362

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant protein of human CD54

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC, IF/ICC

• Dilution :

  WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100)

• Purity :

  The antibody was purified by immunogen affinity chromatography.

• Modification :

  Unmodification

• Molecular Weight :

  ~ 95 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Recognizes endogenous levels of CD54 protein.

• Applications Notes :

  Western blot analysis of CD54 expression in A549 IFN-a treated (A), A549 IFN-r treated (B) whole cell lysates.

• Host or Source :

  Rabbit