SOS2 polyclonal antibody

The superfamily of GTP-binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. Studies have identified a family of guanine nucleotide-releasing factors (GRFs) that activate Ras in mammalian cells and an “adapter” protein (Sem 5/GRB2) that appears to mediate the interaction of GRFs with activated receptor molecules. Ras-GRF p140 promotes nucleotide exchange on Ras p21s but not on other members of the Ras gene superfamily. In addition, three mammalian homologs of the Drosophila Ras-GRF, son of sevenless (Sos), have been described. These include two from mouse, mSos 1 and mSos 2, and one from human, hSos. Vav p95 has been reported to function as a GRF in activation of Ras by the T cell receptor and has been reported to have a domain similar to that of Dbl p115, which is a GRF specific for Cdc42Hs. Subsequent to activation, Ras appears to interact with Raf, thereby activating the MAP kinase phosphorylation pathway.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Son of sevenless homolog 2; SOS-2

Q07890

Human, Mouse, Rat

Synthetic peptide, corresponding to amino acids 671-716 of Human SOS2.

Unconjugated

SAPK JNK Signaling Cascades

WB ICC

WB: 1:500~1:1000 ICC: 1:50~1:200

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Unmodification

~ 153 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

SOS2 polyclonal antibody detects endogenous levels of SOS2 protein.

Western blot (WB) analysis of SOS2 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate