ARHGDIG polyclonal antibody

CAT: 0384-BS5899-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS5899-01Size:50 µL
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Background
The Ras superfamily of small GTP-binding proteins are critical mediators of diverse cell signaling pathways, including those leading to proliferation, cytoskeletal organization and secretion. The counter-conversion of the active GTPbound form of these proteins to their inactive GDP-bound form is influenced by two types of regulatory proteins: those that alter the intrinsic GTPase activity of the GTP-binding proteins and those that alter the rate of GDP/GTP exchange. Guanine nucleotide-releasing factors (GRFs) increase the GDP dissociation rate, while GDP-dissociation inhibitors (GDIs) decrease the dissociation rate. The Rho GDI subfamily is composed of Rho GDIα, Ly-GDI (also known as Rho GDIβ and previously known as GDI/D4) and Rho GDIγ. The Rho GDI proteins interact with and have varying affinities for several Ras-like GTP binding proteins, including Rho A, Rho B, Rac and Cdc42. Ly-GDI is expressed only in hematopoietic cells, predominantly in B and T lymphocyte cell lines.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Rho GDP-dissociation inhibitor 3; Rho GDI 3; Rho-GDI gamma; Rho GDIγ; Rho-GDIγ
Swiss Prot
Q99819
Reactivity
Human, Mouse
Immunogen
Synthetic peptide, corresponding to amino acids 176-221 of Human ARHGDIG.
Conjugation
Unconjugated
Applications
WB ICC
Dilution
WB: 1:500~1:1000 ICC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 25 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ARHGDIG polyclonal antibody detects endogenous levels of ARHGDIG protein.
Applications Notes
Western blot (WB) analysis of ARHGDIG polyclonal antibody at 1:500 dilution Lane1:Jurkat cell lysate Lane2:Hela cell lysate Lane3:Raw264.7 cell lysate
Host or Source
Rabbit

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