CD14 polyclonal antibody

• Background:

  Coreceptor for bacterial lipopolysaccharide.In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) .Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response . Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway .Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- .

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14; Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form

• Swiss Prot:

  P08571

• Reactivity:

  Human

• Immunogen:

  Recombinant protein of human CD14.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:5000~1:10000 IF 1:100 - 1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~45 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD14 polyclonal antibody detects endogenous levels of CD14 protein.

• Applications Notes:

  Western blot (WB) analysis of CD14 polyclonal antibody at 1:5000 dilution Lane1:MCF-7 cell membrane lysate (25ug) Lane2:A549 cell membrane lysate (25ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4