CD14 Protein

• Background:

  CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage. Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein. LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection. The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage.

• Swiss Prot:

  P08571

• Modification Site:

  NdeI-XhoI

• Expression System:

  Pet-22b (+)

• Tag:

  His-tag

• Purity:

  Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

• Solubility:

  PBS, 4M Urea, PH7.4

• Molecular Weight:

  ~40kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Host or Source:

  E.coli

• AA Sequence:

  ACCACCCCTGAACCGTGCGAACTGGATGATGAAGACTTCCGCTGCGTGTGTAACTTCAGTGAACCGCAGCCGGATTGGAGCGAAGCCTTCCAGTGCGTTAGTGCAGTTGAAGTGGAAATTCATGCAGGCGGCCTGAATCTGGAACCGTTCCTGAAACGCGTTGATGCAGATGCAGATCCGCGCCAGTATGCCGATACCGTTAAAGCCCTGCGTGTGCGTCGTCTGACCGTGGGTGCAGCACAGGTGCCGGCACAGCTGCTGGTTGGTGCACTGCGTGTTCTGGCCTATAGTCGCCTGAAAGAACTGACCCTGGAAGATCTGAAAATTACCGGTACCATGCCGCCGCTGCCGCTGGAAGCCACCGGTCTTGCACTGAGCAGCCTGCGTCTGCGTAATGTGAGCTGGGCCACCGGTCGCAGCTGGCTGGCAGAACTGCAGCAGTGGCTGAAACCGGGTCTGAAAGTTCTGAGCATTGCCCAGGCACATAGCCCGGCATTCAGTTGCGAACAGGTTCGCGCCTTCCCGGCACTGACCAGTCTGGATCTGAGTGATAATCCGGGTCTGGGCGAACGCGGTCTGATGGCCGCACTGTGTCCGCATAAATTCCCGGCAATTCAGAATCTGGCACTGCGTAATACCGGTATGGAAACACCTACCGGTGTGTGTGCAGCCCTGGCCGCCGCTGGTGTGCAGCCTCATAGCCTGGATCTGTCACATAATAGCCTGCGCGCCACCGTTAATCCGAGCGCCCCGCGTTGCATGTGGAGCAGCGCACTGAATAGCCTGAATCTGAGCTTCGCAGGTCTGGAACAGGTTCCGAAAGGCCTGCCGGCAAAACTGCGTGTTCTTGATCTGAGCTGTAATCGCCTGAATCGCGCACCGCAGCCGGACGAACTGCCGGAAGTGGATAATCTGACCCTGGATGGTAATCCGTTCCTGGTGCCGGGCACCGCACTGCCTCATGAAGGCAGCATGAAT