CD142 polyclonal antibody

• Background:

  Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Tissue factor; TF; Coagulation factor III; Thromboplastin; CD142

• Swiss Prot:

  P13726

• Reactivity:

  Human

• Immunogen:

  Recombinant protein of human CD142.

• Conjugation:

  Unconjugated

• Applications:

  WB IP IF

• Dilution:

  WB: 1:2000~1:5000 IF 1:50~1:200 IP 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 42 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD142 polyclonal antibody detects endogenous levels of CD142 protein.

• Applications Notes:

  Western blot (WB) analysis of CD142 polyclonal antibody at 1:2000 dilution Lane1:A549 cell membrane lysate (25ug) Lane2:L02 cell membrane lysate (25ug) Lane3:EC9706 cell membrane lysate (25ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4