CD14 monoclonal antibody

• Background:

  CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage. Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein. LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection. The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage .

• Structure Composition:

  Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

• Product Name Alternative:

  Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14)

• Swiss Prot:

  P08571

• Reactivity:

  Human

• Immunogen:

  Native purified human CD14.

• Conjugation:

  Unconjugated

• Applications:

  IF, FC

• Dilution:

  IF (1/50 - 1/200)

• Purity:

  The monoclonal antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 40 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes human CD14

• Applications Notes:

  Flow cytometric analysis of human peripheral blood monocytes using Anti-CD14 Antibody, followed by anti-mouse IgG PE.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4